| 摘要 |
The present invention provides means and methods for the generation and manufacturing of recombinant Ad vectors that are modified in E2B and/or E4 functions, preferably, said vectors comprise E1 and/or E2A deletions. For th is purpose, the vector genome is modified in the respective promoter regions su ch that the promoter is only active in a suitable complementing cell line or on ly active following a certain signal in the case of an inducible promoter. The modified promoter is, on the other hand, inactive under normal conditions, a nd in normal mammalian and/or human cells. Hence, vectors that possess said modified promoter in the E2B and/or E4 region do not express the respective transcription region in mammalians and/or humans.
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