| 摘要 |
<p>The invention concerns a method for moving, isolating and/or identifying particles in a sample by placing said sample in a spatially varied electrical field which acts independently and selectively on charged, dipolar and higher moments of the particle in a medium. The invention presented herein, comprises several novel methods of utilisation of the effect of nonlinear mobility and non-uniform electric field for preparative separation and improved analysis of macromolecules in a large variety of sizes and especially in the separation of DNA and its fragments and other biological molecules. Specifically, as demonstrated herein, the present invention uses nonlinear corrections of molecular mobility in an electric field in media, which are considered as important parameters for improved Separation and peak narrowing. The invention also provides an electrophoresis System which separates charged biological macromolecules by means of applying a non-uniform electrical potential across a buffer of electrolyte solution and supported gel, which contain those molecules. The system of the invention includes a power supply and control system which has a wide dynamic range of constant voltage, current and power which may be supplied, and is therefore particularly suited to the needs of the electrophoresis system.</p> |
