| 摘要 |
There is disclosed a substantially pure peptide comprising the following sequence: [ML] -X(3) -[LIVMC] -X(3) -[GMNEHK] -[MLFYI] -X(2) -[ST] -X(3) -[SAIG] - [RMIK] -X(2) -[MIVA] -X(2) -[GNRHK] -X-[IVLF] -X-[LIV] -[NDSRGAL] -X(3) -[LIVSQ] -X(2) -[PILVTAC] wherein X represents any amino acid. Also disclosed is a method for determining whether a nucleic acid comprises a tRNA anticodon stem-loop. Another aspect comprises a method for identifying a substance which binds to a wherein the binding is detected by assaying for conjugates, for free substance, or for non-complexed peptide. Also described is a method of determining whether a test compound is an agonist or antagonist of a tRNA synthetase-tRNA anticodon stem-loop interaction or ribosomal S4 protein-tRNA anticodon stem-loop interaction. There is also described a method for obtaining a substantially pure nucleic acid comprising a tRNA anticodon stem-loop from a mixture of different nucleic acids. Finally, there is described a pharmaceutical composition for inhibiting the interaction of a tRNA, with a tRNA synthetase or ribosomal protein along with other related aspects.
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