| 摘要 |
PCT No. PCT/GB96/01416 Sec. 371 Date Nov. 7, 1997 Sec. 102(e) Date Nov. 7, 1997 PCT Filed Jun. 14, 1996 PCT Pub. No. WO97/00325 PCT Pub. Date Jan. 3, 1997Mutations in the spoIII gene of B. subtilis abolish sporulation by preventing partition of a prespore chromosome into the small polar prespore compartment. The invention provides a Bacillus strain having a chromosome with two reporter genes each linked to a promoter and responsive to the action of sigma F during sporulation, one located inside and the other located outside a segment of the DNA that is trapped in a prespore compartment; and use of the strain in a method of determining whether an agent inhibits SpoIIIE function in Bacillus species.
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