| 摘要 |
<p>Methods for the preparation of oligonucleotides having bioreversible phosphate blocking groups are disclosed. In one aspect, the present invention provides compounds comprising a sequence of nucleotide units that includes a first segment having at least one internucleoside linkage of the formula: 3'-O-P(X) (O-)-O-5' and a second segment having at least one internucleoside linkage of the formula: 3'-O-P(X) (Y¿1?-(CH2)q-Y2-Y3-Z)-O-5', wherein each of X, Y1, and Y2 is, independently, O or S; q is 2 to about 4; Y3 is C(=O) or S; and Z is aryl having 6 to about 14 carbon atoms or alkyl having from one to about six carbon atoms.</p> |
