| 摘要 |
PROBLEM TO BE SOLVED: To obtain the subject metalloprotease-3 having an angiotensin I- converting activity without causing anaphylaxis, having excellent stability, and useful for medicine for treating herniated intervertebral disk, antitumor medicines, etc. by disposing a specific amino acid sequence. SOLUTION: This metalloprotease-3 has an amino acid sequence of formula I having an active domain at least ranged from the 100-amin acid to the 274-amino acid from the N-terminal and modified with Ile or Tyr as the 172-amino acid residue Xaa, with Va1 or Leu as the 239-amino acid residue Xaa and with continuous His-Ser-Leu or Asn-Ala-Phe as the 241 to 243 amino acid residue Xaa-Xaa-Xaa or an active domain obtained by substituting, deleting or inserting the amino acids of the amino acid sequence. The active domain includes an amino acid sequence of formula II. The modified human matrix metalloprotease-3 is obtained by culturing a host cell transformed with a vector containing a DNA encoding the protease-3. |
