发明名称 |
New triplex forming oligonucleotides and their use in anti-hbv |
摘要 |
A type of new triplex forming oligonucleotide (TFO) which can form triplex DNA when bound to two similar fragments of homopolypurine/homopolypyrimidine sequences. TFOs were designed according to the above structure to bind the DR region and pre-S gene promoter region of HBV, respectively. The 3' end of the TFOs can be monophosphorylated or otherwise chemically modified to increase their stability. Cellular experiments prove that these TFOs can be used as drugs to inhibit HBV and treat hepatitis B. TFO and anti-sense oligonucleotide sequences from the DR or pre-S promoter region of HBV can together bind to target RNA sequences and form a (DNA)2:RNA hetero-triplex structure that results in the more efficient inhibition of HBV. |
申请公布号 |
AU9528798(A) |
申请公布日期 |
1999.05.10 |
申请号 |
AU19980095287 |
申请日期 |
1998.10.19 |
申请人 |
SHANGHAI INSTITUTE OF BIOCHEMISTRY, CHINESE ACADEMY OF SCIEN CE |
发明人 |
CHANG-DE LU |
分类号 |
C12N15/09;A61K31/711;A61K38/00;A61K48/00;A61P31/20;C07H21/00;C12N15/113 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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