发明名称 |
Method for producing 2-keto-L-gulonic acid |
摘要 |
An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid (hereinafter 2KLGA) at high yields from D-sorbitol, which is prepared by transforming, with said expression vector, a microorganism capable of producing L-sorbose at high yields from D-sorbitol, which has no or low 2KLGA-decomposing activity or a host microorganism having, in addition to the above-mentioned properties, no or low L-idonic acid-producing activity; and a process for producing 2KLGA, which comprises culturing said transformant in a medium containing D-sorbitol. According to the present invention, 2KLGA useful for the production of L-ascorbic acid can be produced with ease and in larger amounts by a single operation of culture.
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申请公布号 |
US5888786(A) |
申请公布日期 |
1999.03.30 |
申请号 |
US19970943285 |
申请日期 |
1997.10.03 |
申请人 |
FUJISAWA PHARMACEUTICAL CO., LTD. |
发明人 |
NIWA, MINEO;SAITO, YOSHIMASA;ISHII, YOSHINORI;YOSHIDA, MASARU;HAYASHI, HIROMI |
分类号 |
C12N9/02;C12N9/04;C12N15/53;C12P7/60;(IPC1-7):C12P7/60;C12N15/00;C12N1/00 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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