A composition and column used in HPLC, a method for preparing the composition, and a method for separating different chemicals in a sample

摘要

An alkyl-phenyl bonded a stationary phase for use in reversed-phase liquid chromatography, having five to ten carbon atoms in the alkyl group, is disclosed. The alkyl-phenyl bonded phase has its unique selectivity, and it is chemically stable in both acidic and basic environments as well as havign high retention capacity, hence high resolution. A method for preparing the alkyl-phenyl bonded phase ad a column inside of wihich the alkyl-phenyl bonded phase is packed with are also disclosed. Additionally, a method for performing liquid chromatography and a mixed stationary phase which has more than one alkyl-phenyl phases are disclosed.