MODIFIED POLYPEPTIDES WITH HIGH ACTIVITY AND REDUCED ALLERGENICITY

摘要

The present invention relates to a modified polypeptide which has an enzymatic activity level of greater than about 70 % of the parent polypeptide and an allergenic response level of less than about 33 % of the parent polypeptide. Embodiments of the present invention relate to modified polypeptides with reduced allergenicity and high enzymatic activity comprising the formula: A-Bn, wherein A is an enzyme, and mixtures thereof; B is a twin polymer moiety, having a total molecular weight of from about 0.5 kilodaltons (KD) to about 40 KD, having formula (1), conjugated to the enzyme; wherein R1 and R<2> are essentially straight chain polymers, having a molecular weight ranging from about 0.25 KD to about 20 KD; wherein the ratio of the molecular weights of R1 and R2 is from about 1:10 to about 10:1, wherein X is a linking moiety which links the twin moeity to a single site on the enzyme; and n is the number of twin polymer moietis conjugated to the enzyme, and represents an integer from about 1 to about 15.