NOVEL VECTORS AND EXPRESSION METHODS FOR PRODUCING MUTANT PROTEINS

摘要

The present invention describes a new shuttle vector permitting the expression of engineered toxin mutants and toxin fusion proteins in Corynebacterium. In addition the invention provides a mutant Pichia pastoris, a method for producing this mutant and a method of expressing engineered toxin mutants and toxin fusion proteins in the mutant form of Pichia pastoris. The invention further provides a mutant Chinese hamster ovary (CHO) cell, a mutant insect cell, a method for producing these mutants and a method of expressing engineered toxin mutants and toxin fusion proteins in the mutant cells.