Detection of d genome in wheat species

摘要

A method for detecting the presence of wheat having a D-genome in for example a processed food product is described. The method comprises extracting DNA from said product and detecting the presence of a nucleotide sequence which is characteristic of the D-genome and in particular is derived from the Dgas44 sequence or from the PSR128 intron. The sequence is suitably detected using PCR. A particular sequence within each of the Dgas44 and the single copy PSR128 intron has been identified as being a suitable target for detection. The method is useful in detecting contamination of flour, semolina, or pasta products which are made from T. durum by bread wheat. Reagents useful in the method as well as kits for conducting the method are also described and claimed.