| 摘要 |
PROBLEM TO BE SOLVED: To activate a protein of NT-3 (neurotrophin 3) after expression by treating an inclusion body obtained by expressing a protein into a prokaryotic cell host by technique of generic engineering under specific conditions. SOLUTION: An insoluble and inactive inclusion body obtained by expressing a protein into a prokaryotic cell host by technique of genetic engineering is ground and solubilized with a modifier (e.g. urea) and the modifier is removed and modifying action of a residual modifying agent is lost by an acidic solution (pH1-4) such as hydrochloric acid. Further, a precipitation-preventing agent (e.g. saccharide) for suppressing insolubilization is added thereto and a metal salt such as copper sulfate is added thereto at pH about 4 to 7. |
