| 摘要 |
PROBLEM TO BE SOLVED: To rapidly and conveniently measure the subject enzyme in an excellent accuracy by adding a maltooligoside derivative inert to an exo type glycolytic enzyme and a conjugated enzyme system to a sample containing a specific amylase originating from a brewed product and then determining the compound liberated after completion of the reaction. SOLUTION: This method for measuringα-amylase comprises adding a maltooligoside derivative of the formula [(n) is 2-5; R is an aromatic coloring moiety; X is an azide, a halogen, an alkyl or aryloxy, an alkyl or arylsulfonyloxy and a carbamoyloxy; Y is H, an alkyl, an alkyl- or arylsulfonyl, a carbamoyl, etc.] not cleaved by an exo type sugar hydrolase and a conjugated enzyme system such as glucoamylase or the like to a specimen containingα-amylase arising from a brewed product and having an optimum pH of 3 0-5.0 to cause an enzyme reaction and finally determing a liberated compound such as an aromatic coloring compound or the like. The objectiveα-amylase is quite conveniently measured in a short time and in an excellent accuracy (sensitivity).
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