摘要 |
<p>Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, β-lactoglobulin 1 and β-lactoglobulin 2 on the anion exchanger (I) is higher than the elution ionic strength required for a quaternary comparative ion exchanger. Chromatofocusing and separation on ECTEOLA-cellulose is not included. New anion exchangers which are according to structure (I) wherein R1-6 are hydrocarbon groups which can contain ether oxygen atoms, hydroxyl groups, and different types of amino groups. X is OH or alkoxy or amino. B is a ligand arm replacing a group R1-6 or a hydrogen in any of R1-6. If one of the groups R5 and R6 contains a nitrogen atom then one of the groups also contains a hydroxy or alkoxy group. ECTEOLA-cellulose and variants where both R5 and R6 are selected among methyl and ethyl are not included.</p> |