A positive selection vector is provided useful for the transformation and screening of Gram positive bacteria and particularly Bacillus sp. for the presence of foreign DNA. The vector comprises a mutant gene encoding a signal peptide processing mutation. Expression of the mutant gene in a Bacillus host cell which lacks the ability to metabolize sucrose is lethal when cells are grown in the presence of sucrose. Foreign DNA may be inserted into the vector so as to inactivate the mutant gene thereby permitting the cells to grow in the presence of sucrose and allowing for facile selection of transformants.
申请公布号
WO9716558(A1)
申请公布日期
1997.05.09
申请号
WO1996US17636
申请日期
1996.10.31
申请人
E.I. DU PONT DE NEMOURS AND COMPANY;BRAMUCCI, MICHAEL, GENE;NAGARAJAN, VASANTHA