PREPARATION OF PHARMACEUTICAL GRADE HEMOGLOBINS BY HEAT TREATMENT IN PARTIALLY OXYGENATED FORM

摘要

During the purification of pharmaceutical grade cross-linked hemoglobin mixtures of cross-linked and uncross-linked hemoglobin are heated in the presence of nonstoichiometric amounts of oxygen, resulting in the selective precipitation of the uncross-linked hemoglobin. After separation of the precipitated uncross-linked tetramers, the cross-linked hemoglobin remaining in the supernatant is so purified that a further chromatography purification step is unnecessary. This hemoglobin is highly cross-linked, absolutely free of chromatography fines, and has a low methemoglobin content.