发明名称 |
PROCESS FOR THE TRANSCRIPTIONLESS AMPLIFICATION OF NUCLEIC ACIDS |
摘要 |
The present invention relates to a process for the transcriptionless amplification of nucleic acids or nucleic acid sequences by means of enzymes in which the nucleic acids or sequences are divided at least partially into their individual strands before amplification and/or transcribed and the reaction mixture contains oligonucleotides with a base sequence essentially complementary to those of the ends of the nucleic acids or sequences to be amplified, where said oligonucleotides can, in certain conditions, form starting points and/or chemical components for the synthesis of nucleic acids and are built into the amplification product to be formed. The product of the components itself again corresponds to the nucleic acid or sequence to be amplified. The reaction product formed from the oligonucleotide components, possibly from the templates and other chemical components, reacts again in one step with the oligonucleotide components, the reaction being conducted substantially isothermally. |
申请公布号 |
WO9700330(A2) |
申请公布日期 |
1997.01.03 |
申请号 |
WO1996AT00106 |
申请日期 |
1996.06.13 |
申请人 |
HIMMLER, GOTTFRIED;SCHLEDERER, THOMAS |
发明人 |
HIMMLER, GOTTFRIED;SCHLEDERER, THOMAS |
分类号 |
C12N15/09;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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