发明名称 |
Multi-domain DNA ligands for protein and nucleic acid affinity chromatography and processing of solid-phase DNA |
摘要 |
The present invention is directed to a multi-domain oligonucleotide ligand having multiple functions conferred by each of the domains. The ligand is bound to a solid phase matrix for use in both mRNA-affinity chromatography and as a priming matrix for generating matrix-bound cDNA from the mRNA bound to the matrix. The resultant cDNA was tested and found to function as a solid-phase template for PCR. This solid-phase template approach to PCR is demonstrated with oligo-d(T) paper. The functional domains are defined by polynucleotide sequences on the ligand to confer the different functions.
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申请公布号 |
US5559001(A) |
申请公布日期 |
1996.09.24 |
申请号 |
US19950478049 |
申请日期 |
1995.06.07 |
申请人 |
ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF THE UNIVERSITY OF ARIZONA |
发明人 |
UTERMOHLEN, JOSEPH G. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C07H21/02 |
主分类号 |
C12Q1/68 |
代理机构 |
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