摘要 |
Glycoconjugate antigens are prepared by preparing a hapten glycoside, especially an alpha glycoside prepared by the Fischer method with an olefinic aglycon moiety, especially one having a non-terminal double bond, ozonolyzing the hapten glycoside with an olefinic aglycon moiety having a non-terminal double bond to form a hapten-glycoside derivative, preferably without producing Germaldehyde as a by-product, removing by-products of ozonolysis, and conjugating the hapten-glycoside derivative to a carrier.
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