发明名称 |
Method for cloning and producing the BglII restriction endonuclease and modification methylase |
摘要 |
The present invention is directed to a method for cloning and producing the BglII endonuclease by: 1) introducing the restriction endonuclease gene from Bacillus globigii into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the BglII restriction endonuclease activity; and 3) purifying the BglII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the BglII restriction endonuclease.
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申请公布号 |
US5434068(A) |
申请公布日期 |
1995.07.18 |
申请号 |
US19930017664 |
申请日期 |
1993.04.09 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
BROOKS, JOAN E.;HEITER, DANIEL F.;ANTON, BRIAN P. |
分类号 |
C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/16;C12N9/22;C12N15/00;C12N15/09;C12N15/54;C12N15/55;C12R1/07;C12R1/19;(IPC1-7):C12N9/22;C12N15/63 |
主分类号 |
C12N1/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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