| 摘要 |
<p>PCT No. PCT/DK91/00120 Sec. 371 Date Jun. 10, 1991 Sec. 102(e) Date Jun. 10, 1991 PCT Filed May 6, 1991 PCT Pub. No. WO91/17422 PCT Pub. Date Nov. 14, 1991.An apparatus and a method for analyzing particles suspended in a liquid are described, which particles are of a type or are prepared so as to emit radiation characteristic of the particles when exposed to radiation. Particular fluorescence-photometrical flow cytometry is described in connection with biological cell populations. The apparatus comprises an elongated flow area in the shape of a channel provided in a cuvette comprising a first body member having a planar facial surface and a second body member having a planar facial surface. The first and the second body members are adapted to be moved relative to one another between a first position, in which the planar facial surfaces are in fluid-tight contact with one another, and a second position, in which the planar facial surfaces are spaced apart. At least one of the planar facial surfaces of one of the body members is provided with an elongated groove. In the first position, the first and second body members together define the cuvette in which the elongated flow area is defined by the elongated groove constituting a capillary passage. An inlet for feeding the liquid to the elongated flow area is provided in one of the body members and an outlet for discharging the liquid from the elongated flow area is provided in the very same or in the other body member. At least one of the body members is transparent to light to which the particles are exposed and further transparent to the radiation emitted by the particles. Two electrodes are further described for carrying out an election of particles suspended in the liquid by means of a measurement of the electrical conductivity of the fluid, which particles are of a specific size for subsequent registration of fluorescence when exposed to radiation.</p> |
