发明名称 |
Non toxic compositions and methods useful for the extraction of nucleic acids |
摘要 |
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
|
申请公布号 |
US5393672(A) |
申请公布日期 |
1995.02.28 |
申请号 |
US19930156519 |
申请日期 |
1993.11.23 |
申请人 |
MICROPROBE CORPORATION |
发明人 |
NESS, JEFFREY V.;CIMLER, B. MELINA;MEYER, JR., RICH B.;VERMEULEN, NICOLAAS M. J. |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):G01N33/50 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|