| 摘要 |
<p>Method and device for concentrating macromolecules from a solution by means of spinning a concentrator device in a centrifuge. Said device comprises a sample reservoir (1) for receiving a sample to concentrate or purify, filter means for filtering the sample, a centrifuge tube (5) for collecting the filtrate and a concentrate pocket (4) for retention of concentrate. Typically the sample reservoir (1) of the device is provided with a portion constituting a filtration chamber (3) with a volume being only a small fraction of the total volume of the sample reservoir and the filter means is arranged in the sidewall and the concentrate pocket (4) is arranged in the bottom wall of said filtration chamber. In the method the liquid sample is put into the sample reservoir and the unit comprising the sample reservoir (1) and the filter means (2) is put at least partly into the centrifuge tube for collecting the filtrate. This assembly is centrifuged whereby a force vector is created acting on the macromolecules and sweeping the filter surface, the macromolecules are collected in a concentrate pocket (4) away from the filter surface thereby eliminating the risk of concentration to dryness.</p> |
