METHOD FOR PURIFYING HUMAN PARVOVIRUS STRUCTURAL PROTEIN

摘要

PURPOSE:To purify a human parvovirus structural protein by comprising at least two steps of an isoelectric focusing step, an anion exchange step and a hydrophobic chromatographic step. CONSTITUTION:A purifying step comprising at least two steps [with the proviso that the combination of (A) with (B) is removed] in the following three steps (A), (B) and (C) is applied in order to obtain a high-purity human parvovirus structural protein from Escherichia coli producing the human parvovirus structural protein: step (A) (isoelectric focusing): a solution containing the human parvovirus structural protein is subjected to the isoelectric focusing to recover a fraction at pH 6-8; step (B) (anion exchange step): the adsorption with an anion exchanger and elution with a solution at 0.05-0.6 M inorganic salt concentration and step (C) (hydrophobic chromatographic step): the adsorption on a hydrophobic chromatographic support and elution with a solution at <=0.3M inorganic salt concentration or a solution containing 5-70 (vol./vol.)% organic solvent.