| 摘要 |
The invention relates to a microbiologically produced, heat-stable N-acyl-L-proline acylase and to a process for obtaining it from Comamonas testosteroni DSM 5416 or Acaligenes denitrificans DSM 5417. The enzyme can be used to obtain L-proline from N-acetyl-D,L-proline, N-chloroacetyl-D,L-proline, N-formyl-D,L-proline, N-propionyl-D,L-proline, N-butyryl-D,L-proline, N-valeryl-D,L-proline, N-caproyl-D,L-proline, to obtain L-thiazolidine-4-carboxylic acid from N-acetyl-D,L-thiazolidine-4-carboxylic acid or N-chloroacetyl-D,L-thiazolidine-4-carboxylic acid, to obtain L-thiazolidine-2-carboxylic acid from N-acetyl-D,L-thiazolidine-2-carboxylic acid or N-chloroacetyl-D,L-thiazolidine-2-carboxylic acid, and L-pipecolic acid from N-acetyl-D,L-pipecolic acid or N-chloroacetyl-D,L-pipecolic acid. The enzyme can also be employed to produce N-acetyl-L-proline, N-propionyl-L-proline and N-butyryl-L-proline from L-proline and the relevant carboxylic acid. |
