发明名称 |
METHOD AND MATERIALS FOR INTRODUCING DNA INTO PREVOTELLA RUMINICOLA |
摘要 |
2110924 9221761 PCTABS00017 A method of introducing expressible heterologous DNA into Prevotella ruminicola is provided. The method involves conjugal transfer of a shuttle vector comprising the heterologous DNA operatively linked to a promoter functional in P. ruminicola. The invention also provides shuttle vectors for use in the method and P. ruminicola produced by the method. The invention further provides a tetracycline resistance gene of the TetQ class, or fragments thereof that confer tetracycline resistance, and a protein of the TetQ class that provides resistance to tetracycline by protecting ribosomes from tetracycline, or active fragments thereof. Finally, the invention provides a promoter functional in P. ruminicola) and an engineered P. ruminicola comprising expressible foreign DNA.
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申请公布号 |
CA2110924(A1) |
申请公布日期 |
1992.12.10 |
申请号 |
CA19922110924 |
申请日期 |
1992.06.01 |
申请人 |
BIOTECHNOLOGY RESEARCH AND DEVELOPMENT CORPORATION;BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS |
发明人 |
SALYERS, ABIGAIL A.;SHOEMAKER, NADJA B.;NIKOLICH, MIKELJON P. |
分类号 |
C07K14/195;C12N1/21;C12N15/64;C12N15/74;(IPC1-7):C12N15/74;C12N15/11;C12N15/31 |
主分类号 |
C07K14/195 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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