REINIGUNGSMETHODE FUER ANTIKOERPER.

摘要

A process for purifying I(g)G3 antibody comprises (a) contacting a liquid contg. I(g)G3 antibody with an affinity matrix which is maintained at a pH of at least 9 and no greater than 9.6 to adsorb I(g)G3 antibody, (b) releasing adsorbed I(g)G3 from the affinity matrix and (c) collecting released I(g)G3 antibody in an eluate at pH 9-9.6. The adsorbed I(g)G3 may be released by contact with a releasing agent and the released I(g)G3 and releasing agent may be sepd. in a desalting matrix. Also claimed is a chromatographic column for purifying I(g)G3 antibody comprising a column contg. an affinity matrix and a desalting matrix for receiving material released from the affinity matrix, the column being equilibrated to a pH of 9-9.6. The affinity matrix may be immobilised protein A. Also claimed is a soln. comprising an aq. soln. contg. I(g)G3 antibody at pH 9-9.6.