| 摘要 |
<p>A method of modifying a plant to increase vegetative growth of commercially valuable plant structures at the expense of non-essential and non-commercial structures is provided. A cDNA library of reproductive-bud mRNA of P. radiata is produced and screened against cDNA prepared from vegetative buds, and non-hybridising colonies were selected. Candidate clones were then selected for the presence of genes expressed in both male and female buds and which expressed on induction by gibberellin A4/7. One clone contained a cDNA corresponding to a gene having the characteristic of early appearance and highly specific expression in male and female reproductive bud development, and the corresponding genomic DNA sequence including the tissue specific promoter of the gene was obtained. The gene was modified by fusing a portion of the gene including the tissue-specific promoter of the gene with a structural gene for a ribonuclease. Candidate gene constructs were used to transform Pinus radiata shoot forming tissues. Explants from successful transformants were screened for expression of the ribonuclease gene upon induction.</p> |
