| 摘要 |
of the disclosure A method for the detection and stepless quantification of substances of diagnostic relevance is indicated, in which a single sample mixture suffices for examination of the sample. Assays which can be used are ELISA, RIA, nucleic acid hybridization, nephelometry and other methods of determination. Examples of substances of diagnostic relevance which are detected are antigens, antibodies of various immunoglobulin classes, nucleic acids or metabolic products of medical importance. The extinction of a sample in the selected assay dilution with colored, enzymatically labeled antigen/antibody complexes is measured, and the titer is calculated from the resulting signal AOD using the formula: Log titer = alpha . <IMG> and can be set equal, with satisfactory accuracy, to the final dilution titer obtainable by serial dilutions. The values for alpha and beta are established experimentally in separate series of tests.
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