PROCESS FOR PRODUCING CDNA

摘要

The process includes the steps of: a) contacting a liquid containing mRNA with an insoluble support having DNA probes attached thereto via the 5'-terminus thereof whereby the mRNA is hybridised to said probes and hence to said support; b) removing said liquid; and c) adding enzymes and nucleotides in solution whereby the probe functions as a primer to produce single stranded cDNA on the mRNA templates. The DNA probe is oligo-dT or a specific DNA sequence which may correspond to a conserved region in a class of mRNA molecules. The insoluble support may consist of magnetic particles which are monodisperse and/or superparamagnetic.