| 摘要 |
PURPOSE:To obtain the subject sarcosine oxidase having excellent heat stability and small Km-value by generating glycine, formaldehyde and hydrogen peroxide from sarcosine, water and oxygen and making resultant compound to have specific stable pH, optimum pH, heat stability and optimum temperature, Km- value and molecular weight. CONSTITUTION:The subject sarcosine oxidase is an enzyme has following properties: 1) acts as a catalyst of the reaction: sarcosine+H2O+O2 glycine+ formaldehyde+H2O2, 2) stable pH: about 6.5-9.0, 3) optimum pH: about 7.0-8.5, 4) heat stability: below 55 deg.C, 5) optimum temperature: about 40-50 deg.C, 6) Km-value for sarcosine: 2.8X10<-3> M, 7) molecular weight: about 65000. Said enzyme is obtained by culturing Arthrobacter fungus producing sarcosine oxidase, preferably Arthrobacter sp-TE 1826 (FERM P-10637) in a nutritive medium and extracting from inside of the fungus cell. |
