| 摘要 |
PURPOSE:To produce L-alanine in high yield without manifesting racemase activity by enzymatically reacting L-aspartic acid in the presence of a bacterium cell having enzymatic activity while maintaining pH 4.3-50 and 40-47 deg.C. CONSTITUTION:Pseudomonas dacunhae IAM1152 strain containing L-aspartic acid beta-decarboxylase is aerobically cultured in a medium containing a carbon source such as fumaric acid, a nitrogen source such as ammonium and an inorganic salt useful for ordinary microorganisms and the prepared cell is used for enzymatic reaction. Then an aqueous solution containing L-aspartic acid or a salt thereof is adjusted to proper pH in the presence of the bacterium cell and subjected to enzymatic reaction at pH 4.3-5.0 at 40-47 deg.C. Then separation and purification of formed alanine in the reaction solution can be carried out by well-known ion exchange resin treatment, etc. |
