ANALYTICAL METHOD AND APPARATUS FOR DETERMINING FLUORESCENCE OR PHOSPHORESCENCE

摘要

A turnable holding a train of reaction containers thereon is continuously turned by one revolution plus an angle for a share of one container and then stopped. This makes one cycle. On the route of transfer of the reaction container, there are provided a light irradiation position and a light collection position. …The reaction container into which a sample and a marker reagent have been introduced is transferred such that it does not stop at the irradiation position and the light collection position. The reaction container to which a monochromatic light for excitation has been irradiated at the irradiation position is transferred toward the light collection position. Fluorescence coming from the light collection position is lead to a photodetector, and the concentration of the target substance in the sample is determined from the detected fluorescence after arithmetic processing. …Since the light collection position is provided at a place different from the irradiation position in the present apparatus, it is possible to measure fluorescence originating from the sample which is substantially free from the influence of background fluorescence.