| 摘要 |
The novel L-sorboson-dehydrogenase according to the invention catalyses the oxidation of L-sorboson to 2-keto-L-gulonic acid in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as a coenzyme. 2-Keto-L-gulonic acid is an important precursor in the preparation of vitamin C. …<??>The process according to the invention for the preparation of the novel L-sorboson-dehydrogenase is carried out by culturing a microorganism belonging to the genus Gluconobacter, or a mutant of this microorganism, which is capable of producing the novel L- sorboson-dehydrogenase in cell material, breaking up the cell material, isolating the L-sorboson-dehydrogenase from cell-free extract of the broken-up cell material, preferably from the cytosol fraction of the cell material, and purifying the L-sorboson- dehydrogenase. |
