| 摘要 |
NEW MATERIAL:An biologically active polypeptide having amino acid sequence expressed by the formula. USE:An antimicrobial agent and antivirus agent. PREPARATION:For example, established cell line NIH Sape-4 derived from Sarcophaga peregrina embryo is planted in a culture medium subjected to sterilization treatment, cultivated at 25 deg.C for 7-10 days and passage is carried out when the cell numbers attain 2X10<7>/ml and supernatant is recovered by centrifugation. Then, a phosphoric acid buffer of 6.0pH is added to the supernatant to control the pH and slat concentration and the solution is passed through a CM cellulose column to subject the solution to adsorption treatment and after the column is washed, 520M NaCl-containing phosphoric acid buffer is passed through the column and the eluate is purified by a column chromatography to provide the compound expressed by the formula. |
