| 摘要 |
1,224,744. Determination of hydrogen peroxide. MERCK PATENT G.m.b.H. 28 Feb., 1968 [10 March, 1967; 9 May, 1967], No. 9617/68. Heading B1X. A reagent for use in determination of hydrogen peroxide, which may be formed in situ by enzyme reaction, comprises a water soluble vanadate, a water soluble iodide and a chromogen which reacts with iodine to produce a colour change in the solution. The chromogen may be di-anisidine, o-tolidine, anisidine, ptoluidine, o-phenylene-diamine, benzidine, 2,7- diamino-fluorene, o-toluidine, alpha-naphthol, guaiacol, preferably in salt form. The reagent may also contain a phosphate or tris buffer to provide a pH of 4.5 to 9.5, a protective colloid which is preferably first freed from peroxides, and, for determining enzymes or enzyme substrates, the appropriate substrate or enzyme respectively. In determining glucose oxidase or galactose oxidase, the test solution is preferably saturated with pure oxygen. The reagent may be freeze dried and reconstituted for use, or used as powder, tablets or impregnated test paper. Examples relate to testing solutions comprising hydrogen peroxide, peroxydisulphate glucose oxidase, galactose oxidase, galactose, glucose, d-amino acids, d-amino acid oxidase, and using the reagents as liquid spray for developing paper and thin layer chromatograms. A spray comprising galactose oxidase is used to determine galactose and galactose compounds on a paper or thin layer chromatogram of a mixture of carbohydrates, e.g. a glycoside hydrolysis mixture; a spray comprising glucose oxidase is used to determine glucose on paper or thin layer chromatograms; and a spray containing an excess of d-amino acid oxidase is used to identify d-amino acids in the presence of the corresponding 1-acids on paper or thin layer chromatograms. |
