Process and reagent for the determination of substrates or enzyme activities.

摘要

This process, which uses a redox reaction as the measuring reaction, is carried out in the presence of one or more additionally added tetrazolium salts, which render reducing interfering compounds ineffective. A reagent suitable for this determination and novel tetrazolium salts of the general formula I'

in which
R<1><'> denotes a carboxyl radical, a phenyl radical which is unsubstituted or substituted in the 2 or 4 positions by a carboxyl group, disubstituted in the 2 and 4 positions by nitro groups or in the 4 position by trimethylammonium,
R<2><'> denotes an unsubstituted phenyl radical,
R<3><'> denotes a phenyl radical which is unsubstituted or substituted in the 2 position by a carboxyl group and
A<'> [i.e. A<1><->] denotes a customary counteranion, are also provided.