| 摘要 |
<p>PURPOSE:The titled antibody useful for immunological study, clinical diagnosis, immunological remedy, etc. of cancer, produced from a hybridoma formed by cell fusion of an antibody-forming cell of a lung-cancer patient and a human tumor cell strain. CONSTITUTION:An antibody-forming cell of a lung-cancer patient and a mutant cell (NAT-30 and its substrain) of hypoxanthine-guanine-phosphoribosyltransferase deficiency of Namaruba cell of human Burkitt's lymphoma cell as a cell of human tumor cell strain are fused to form a hybridoma. The hybridoma is cultivated in a serum-free medium or in a bovine fetal serum-containing cell, to give a human monoclonal anti-lung cancer cell antibody which is (i) reacted strongly with a lung-cancer cell, (ii) hardly reacted with cells of stomach cancer, kidney cancer, myeloma, and bladder cancer, and (iii) not reacted with normal cells.</p> |
