Assay for monoclonal antibody against surface Ig of a human B cell tumor

摘要

An assay that facilitates screening of hybridoma culture media for monoclonal anti-idiotype antibodies, particularly murine monoclonal antibodies that are useful for treating human B cell tumors is disclosed. The assay is a solid phase type assay and involves: incubating a lysate of the patient's B cell tumor with immobilized anti-human Ig; separating unbound lysate materials; incubating the remaining immobilized complex with the test culture medium; separating unbound culture medium material; and incubating the remaining immobilized complex with a labeled anti-mouse Ig; separating unbound labeled anti-mouse Ig; and detecting the presence of label in the remaining immobilized complex.