摘要 |
<p>The following are claimed: (A) an alburmin/specific ligand coupling prod. which is soluble in aq. media; (B) a process for immunoassoy of a biological substance (I), comprising (a) immobilising a substance (II) having binding affinity for (I), (b) incubating with a medium contg. (I), (c) washing the resulting reaction mixt. and incubating with an albumin/specific ligand coupling prod. in soln. in an aq. medium, where the ligand is capable of reacting specifically with (I) or (II), (d) washing the resulting reaction mixt. and incubating with a labelled anti-albumin antibody, and (e) detecting the label; (C) an immunoassay test kit comprising an albumin/ specific ligand coupling prod., a labelled anti-albumin antibody and reagents for detecting the label. Coupling with albumin increases sensitivity, esp. in the base of enzyme immunoassays for antigens, haptens or antibodies.</p> |