| 摘要 |
<p>An apparatus for the stepwise synthesis of polynucleotides in which the polynucleotide chains are extended in stepwise fashion from a modified form of polymer support to which the first unit is linked comprises a reaction column containing the polymer supported product and acting as the reaction vessel, and a series of reaction bottles all connected to the reaction column by means of a fluid flow conduit to which the vessels make connection via two-way valves arranged in series. The farthest upstream vessel of the series contains reaction solvent, used for washing purposes and the like. The most downstream of the reaction vessels contain nucleotide reagents. Each of the valves has two separate and discreet fluid flow passageways, the first of which is used exclusively for flow of reagent from its associated vessel into the fluid flow conduit, and the second of which is used exclusively for flow of solvent or reagents from an upstream vessel therethrough, thereby eliminating the possibilities of cross-contamination as a result of reagent residuals left in the dead space of the valve. The valves are biased towards their solvent flow condition. Materials are drawn through the fluid flow conduit and valves by suction, e.g. by means of a downstream pump.</p> |
