| 摘要 |
Aspartic acid is enzymatically produced from ammonium fumarate in the presence of the enzyme, aspartase. A cell-free aspartase solution is prepared by forming an aqueous cell suspension of aspartase- containing microbial cells; incubating the cell suspension, centrifuging the cell suspension to form a clarified solution that contains a major portion of the aspartase activity originally present in the cells, a sufficient amount of an aspartase-stabilizing salt being included in the cell suspension or to the clarified solution, to make the clarified solution hypertonic. Aspartase recovered in this manner is particularly useful in immobilized enzyme reactors for the production of aspartic acid. |
