METHODS FOR DETECTING AND QUANTIFYING OCCULT BLOOD IN A HUMAN SPECIMEN

摘要

A method for detecting occult blood in aqueous samples of human specimens, particularly aqueous suspensions of fecal matter is disclosed. The method comprises acidifying an aqueous test sample, which in the case of fecal matter has been treated with lysozyme to clear the suspension; separating the liquid and solid portions of the test sample and passing the acidified liquid portion through an electro-negatively charged membrane; thereafter removing residual test liquids from the membrane; treating the membrane with a peroxidase indicator and observing the development of color on the treated membrane surface. A method for quantifying occult blood is also disclosed which comprises subjecting an aqueous sample to the disclosed detection method and comparing the color developed with a standardized scale reflecting colors developed by treating samples containing known incremental quantities of hemoglobin in accordance with the detection method of the invention.