ADAPTATION OF CELL LINES TO SUSPENSION CULTURE

摘要

C-11-21-0279 APPLICATION FOR LETTERS PATENT FOR ADAPTATION OF CELL LINES TO SUSPENSION CULTURE Densely packed cells growing rapidly in substrateattached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 .mu. to 500 .mu. in diameter. These aggregates are then transferred to fresh medium in agitated liquid suspension. The cells thereby continue to grow in aggregates whereby a normally anchorage-dependent established mammalian cell line is rapidly adapted to suspension culture.