Assay for proteins and polypeptides

摘要

An assay for proteins or polypeptides in solution using a reagent of Coomassie Brilliant Blue G250 dye in perchloric or hydrochloric acid. Upon mixture of the acidic reagent with a protein sample, the dye couples with the protein and undergoes a color change, following which the absorbance of the mixture can be measured to provide a quantitative analysis for the protein in the sample; the same procedure may be used for sample solutions containing polypeptides. Proteins and polypeptides with molecular weights above about 2000-3000 may be assayed.