| 摘要 |
The ergot akaloids having a cyclol side chain are frequently used in medicine. It is the aim of the invention to obtain the alkaloid ergosine, which was not so easily accessible until now, by a convenient process and thus to supply it for pharmaceutical use. This is achieved with the aid of the strain IMET PA 134 of the species Claviceps purpurea (Fr) Tul. This was isolated from saprophytic alkaloid-free wild types by double mutation with the aid of a special selection route (e.g. on the basis of morphological changes). The said strain forms, in aerobic submerse or emerse culture in liquid nutrient media which contain a carbon source, a nitrogen source and mineral salts, at 20-30 DEG C, pH 4.0 - 6.2 in the course of 10-35 days, an alkaloid mixture of 90% ergosine/ergosinine and 10% clavine alkaloids with a total alkaloid content of 300-350 mu g of alkaloid per ml of nutrient solution. |
