Purification of microbial enzyme extracts using synthetic polyelectrolytes

摘要

Nucleic acids and unwanted proteins are removed from microbial enzyme extracts by precipitation with water-soluble, cationic polymers comprised of monomers having the structure wherein R1 is a hydrogen atom, a lower alkyl group, or the equivalent and R2 is a nitrogen containing group capable of carrying a positive electrical charge when the polymer is dissolved in an aqueous solution.