| 摘要 |
<p>An experimental and culture vessel for biological particles, e.g., cell constituents, cells or aggregates of cells, has filters of defined pore size on the inlet and outlet sides and is traversed vertically, contrary to the direction of gravity, by liquid culture medium. The cross-section of the interior of the vessel varies between these filters such that in steady-state flow the speed of flow of the soln. through the entire area of the inlet filter exceeds the relative sinking speed of the particles w.r.t. the medium, whereas the corresponding flow rate through the outlet filter is smaller than this relative sinking speed. Heavier particles are prevented from settling on the bottom filter and lighter particles from accumulating beneath the top filter; in this way clogging of the filters and progressive growth of particles adjacent to them is avoided. The particles, even over a wide range of size and thus of sinking speed, remain basically suspended in the upward-flowing medium.</p> |
