HIGHLY CONCENTRATED MEDIUM AND METHOD FOR CULTIVATING ANIMAL CELL

摘要

A high-density cell culture medium is claimed in which all the nutrient components of a conventional medium are fortified in a balanced way for the purpose of cultivating anchorage-independent mammalian cells. More specifically, in the medium the glucose and glutamine may be fortified 2.5 times and 5 times respectively and the other nutrient components of the medium may be fortified 5, 10 or 50 times as compared with those concns. in RPMS 1640 medium. Also claimed is a method of growing cells by contacting the cells with the cell culture medium and biochemicals produced by such cells.